Li, J., Bravo, D.S., Upton, A.L. et al. (7 more authors) (2011) Close temporal coupling of neuronal activity and tissue oxygen responses in rodent whisker barrel cortex. European Journal Of Neuroscience, 34. pp. 1983-1996. ISSN 0953-816X
Abstract
Neuronal activity elicits metabolic and vascular responses, during which oxygen is first consumed and then supplied to the tissue via an increase in cerebral blood flow. Understanding the spatial and temporal dynamics of blood and tissue oxygen (To(2)) responses following neuronal activity is crucial for understanding the physiological basis of functional neuroimaging signals. However, our knowledge is limited because previous To(2) measurements have been made at low temporal resolution (>100 ms). Here we recorded To(2) at high temporal resolution (1 ms), simultaneously with co-localized field potentials, at several cortical depths from the whisker region of the somatosensory cortex in anaesthetized rats and mice. Stimulation of the whiskers produced rapid, laminar-specific changes in To(2). Positive To(2) responses (i.e. increases) were observed in the superficial layers within 50 ms of stimulus onset, faster than previously reported. Negative To(2) responses (i.e. decreases) were observed in the deeper layers, with maximal amplitude in layer IV, within 40 ms of stimulus onset. The amplitude of the negative, but not the positive, To(2) response correlated with local field potential amplitude. Disruption of neurovascular coupling, via nitric oxide synthase inhibition, abolished positive To(2) responses to whisker stimulation in the superficial layers and increased negative To(2) responses in all layers. Our data show that To(2) responses occur rapidly following neuronal activity and are laminar dependent.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2011 The Authors. European Journal of Neuroscience © 2011 Federation of European Neuroscience Societies and Blackwell Publishing Ltd. Open Access - Re-use of this article is permitted in accordance with the Terms and Conditions set out at http://wileyonlinelibrary.com/onlineopen#OnlineOpen_Terms |
Keywords: | Action Potentials/drug effects/*physiology Animals Cerebrovascular Circulation/*physiology Electric Stimulation/methods Enzyme Inhibitors/pharmacology Female Indazoles/pharmacology Magnetic Resonance Imaging Male Mice Mice, Inbred C57BL Neurons/drug effects/*physiology Oxygen/*metabolism Physical Stimulation/methods Rats Rats, Sprague-Dawley Somatosensory Cortex/*blood supply/*physiology Vibrissae/*physiology |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Psychology (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 18 Aug 2016 15:10 |
Last Modified: | 18 Aug 2016 15:10 |
Published Version: | http://dx.doi.org/10.1111/j.1460-9568.2011.07927.x |
Status: | Published |
Publisher: | Blackwell Publishing |
Refereed: | Yes |
Identification Number: | 10.1111/j.1460-9568.2011.07927.x |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:103810 |