Sutherland, B., Fordsmann, J., Martin, C.J. orcid.org/0000-0002-8186-784X et al. (8 more authors) (2017) Multi-modal assessment of neurovascular coupling during cerebral ischaemia and reperfusion using remote middle cerebral artery occlusion. Journal of Cerebral Blood Flow and Metabolism, 37 (7). pp. 2494-2508. ISSN 1559-7016
Abstract
Hyperacute changes in cerebral blood flow (CBF) during cerebral ischemia and reperfusion is an important determinant of injury. CBF is regulated by neurovascular coupling (NVC), and disruption of NVC contributes to brain plasticity and repair problems. However, it is unknown how NVC is affected hyperacutely during cerebral ischemia and reperfusion. We have developed a remote middle cerebral artery occlusion (MCAO) model in the rat, which enables multi-modal assessment of NVC immediately prior to, during and immediately following reperfusion. Male Wistar rats were subjected to remote MCAO, where a long filament was advanced intraluminally through a guide cannula in the common carotid artery. Transcallosal stimulation evoked increases in blood flow, tissue oxygenation and neuronal activity, which were diminished by MCAO and partially restored during reperfusion. These evoked responses were not affected by administration of the thrombolytic alteplase at clinically used doses. Evoked CBF responses were fully restored at 24 hours post-MCAO indicating that neurovascular dysfunction was not sustained. These data show for the first time that the rat remote MCAO model coupled with transcallosal stimulation provides a novel method for continuous assessment of hyperacute NVC changes during ischemia and reperfusion, and offers unique insight into hyperacute ischemic pathophysiology.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © Author(s) 2016. This article is distributed under the terms of the Creative Commons Attribution 3.0 License (http://www.creativecommons.org/licenses/by/3.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
Keywords: | Neurovascular coupling; stroke; middle cerebral artery occlusion; cerebral blood flow; alteplase |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Psychology (Sheffield) |
Funding Information: | Funder Grant number ROYAL SOCIETY UF080113 ROYAL SOCIETY UF130327 WELLCOME TRUST (THE) 093223/B/10/Z |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 17 Aug 2016 08:14 |
Last Modified: | 24 Jan 2020 14:56 |
Published Version: | https://doi.org/10.1177/0271678X16669512 |
Status: | Published |
Publisher: | Nature Publishing Group |
Refereed: | Yes |
Identification Number: | 10.1177/0271678X16669512 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:103809 |