Pele, LC, Haas, CT, Hewitt, RE et al. (8 more authors) (2017) Synthetic mimetics of the endogenous gastrointestinal nanomineral: silent constructs that trap macromolecules for intracellular delivery. Nanomedicine: Nanotechnology, Biology and Medicine, 13 (2). pp. 619-630. ISSN 1549-9634
Abstract
Amorphous magnesium-substituted calcium phosphate (AMCP) nanoparticles (75-150nm) form constitutively in large numbers in the mammalian gut. Collective evidence indicates that they trap and deliver luminal macromolecules to mucosal antigen presenting cells (APCs) and facilitate gut immune homeostasis. Here, we report on a synthetic mimetic of the endogenous AMCP and show that it has marked capacity to trap macromolecules during formation. Macromolecular capture into AMCP involved incorporation as shown by STEM tomography of the synthetic AMCP particle with 5nm ultra-fine iron (III) oxohydroxide. In vitro, organic cargo-loaded synthetic AMCP was taken up by APCs and tracked to lysosomal compartments. The AMCP itself did not regulate any gene, or modify any gene regulation by its cargo, based upon whole genome transcriptomic analyses. We conclude that synthetic AMCP can efficiently trap macromolecules and deliver them to APCs in a silent fashion, and may thus represent a new platform for antigen delivery.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 The Author(s). Published by Elsevier Inc. This paper is licenced under Creative Commons Attribution 4.0 International (CC BY 4.0). |
Keywords: | Amorphous magnesium-substituted calcium phosphate; nanoparticles; peptidoglycan; PD-L1 |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemical & Process Engineering (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 04 Aug 2016 14:15 |
Last Modified: | 23 Oct 2017 23:25 |
Published Version: | https://doi.org/10.1016/j.nano.2016.07.008 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.nano.2016.07.008 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:103352 |