Hitchcock, A., Jackson, P.J., Chidgey, J.W. et al. (3 more authors) (2016) Biosynthesis of Chlorophyll a in a Purple Bacterial Phototroph and Assembly into a Plant Chlorophyll–Protein Complex. ACS Synthetic Biology, 5 (9). pp. 948-954.
Abstract
Improvements to photosynthetic efficiency could be achieved by manipulating pigment biosynthetic pathways of photosynthetic organisms in order to increase the spectral coverage for light absorption. The development of organisms that can produce both bacteriochlorophylls and chlorophylls is one way to achieve this aim, and accordingly we have engineered the bacteriochlorophyll-utilizing anoxygenic phototroph Rhodobacter sphaeroides to make chlorophyll a. Bacteriochlorophyll and chlorophyll share a common biosynthetic pathway up to the precursor chlorophyllide. Deletion of genes responsible for the bacteriochlorophyll-specific modifications of chlorophyllide and replacement of the native bacteriochlorophyll synthase with a cyanobacterial chlorophyll synthase resulted in the production of chlorophyll a. This pigment could be assembled in vivo into the plant water-soluble chlorophyll protein, heterologously produced in Rhodobacter sphaeroides, which represents a proof-of-principle for the engineering of novel antenna complexes that enhance the spectral range of photosynthesis.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © American Chemical Society 2016 |
Keywords: | photosynthesis; chlorophyll; bacteriochlorophyll; antenna complex; water-soluble chlorophyll protein; Rhodobacter sphaeroides |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Funding Information: | Funder Grant number BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL (BBSRC) BB/M000265/1 EUROPEAN RESEARCH COUNCIL SYNTHPHOTO - 338895 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 02 Aug 2016 11:20 |
Last Modified: | 30 Nov 2016 13:03 |
Published Version: | http://dx.doi.org/10.1021/acssynbio.6b00069 |
Status: | Published |
Publisher: | American Chemical Society |
Refereed: | Yes |
Identification Number: | 10.1021/acssynbio.6b00069 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:103201 |