Forde, N, Maillo, V, O’Gaora, P et al. (7 more authors) (2016) Sexually dimorphic gene expression in bovine conceptuses at the initiation of implantation. Biology of Reproduction, 95 (4). 92. ISSN 0006-3363
Abstract
In cattle, maternal recognition of pregnancy occurs on Day 16 via secretion of interferon tau (IFNT) by the conceptus. The endometrium can distinguish between embryos with different developmental competencies. In eutherian mammals, X-chromosome inactivation (XCI) is required to ensure an equal transcriptional level of most X-linked genes for both male and female embryos in adult tissues, but this process is markedly different in cattle than mice. We examined how sexual dimorphism affected conceptus transcript abundance and amino acid composition as well as the endometrial transcriptome during the peri-implantation period of pregnancy. Of the 5132 genes that were differentially expressed on Day 19 in male compared to female conceptuses, 2.7% were located on the X-chromosome. Concentrations of specific amino acids were higher in the uterine luminal fluid with male compared to female conceptuses, while female conceptuses had higher transcript abundance of specific amino acid transporters (SLC6A19 and SLC1A35). Of note, the endometrial transcriptome was not different in cattle gestating a male or a female conceptus. These data support the hypothesis that, far from being a blastocyst specific phenomenon, XCI is incomplete before and during implantation in cattle. Despite differences in transcript abundance and amino acid utilization in male versus female conceptuses, the sex of the conceptus itself does not elicit a different transcriptomic response in the endometrium.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 by the Society for the Study of Reproduction, Inc. This article is available under a Creative Commons License 4.0 (Attribution-NonCommercial), as described at http://creativecommons.org/licenses/by-nc/4.0. |
Keywords: | amino acids; endometrium; gene expression; uterine luminal fluid; XCI |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM) > Discovery & Translational Science Dept (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 03 Aug 2016 11:40 |
Last Modified: | 09 Jan 2020 12:01 |
Published Version: | https://doi.org/10.1095/biolreprod.116.139857 |
Status: | Published |
Publisher: | Oxford University Press |
Identification Number: | 10.1095/biolreprod.116.139857 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:103097 |