Papolu, PK, Dutta, TK, Tyagi, N et al. (3 more authors) (2016) Expression of a Cystatin Transgene in Eggplant Provides Resistance to Root-knot Nematode, Meloidogyne incognita. Frontiers in Plant Science, 7. 1122. ISSN 1664-462X
Abstract
Root-knot nematodes (RKN) cause substantial yield decline in eggplant and sustainable management options to minimize crop damage due to nematodes are still limited. A number of genetic engineering strategies have been developed to disrupt the successful plant–nematode interactions. Among them, delivery of proteinase inhibitors from the plant to perturb nematode development and reproduction is arguably the most effective strategy. In the present study, transgenic eggplant expressing a modified rice cystatin (OC-IΔD86) gene under the control of the root-specific promoter, TUB-1, was generated to evaluate the genetically modified nematode resistance. Five putative transformants were selected through PCR and genomic Southern blot analysis. Expression of the cystatin transgene was confirmed in all the events using western blotting, ELISA and qPCR assay. Upon challenge inoculation, all the transgenic events exhibited a detrimental effect on RKN development and reproduction. The best transgenic line (a single copy event) showed 78.3% inhibition in reproductive success of RKN. Our results suggest that cystatins can play an important role for improving nematode resistance in eggplant and their deployment in gene pyramiding strategies with other proteinase inhibitors could ultimately enhance crop yield.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Editors: |
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Copyright, Publisher and Additional Information: | © 2016, Papolu, Dutta, Tyagi, Urwin, Lilley and Rao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. This Document is protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission. |
Keywords: | OC-IΔD86; TUB-1; eggplant,; multiplication factor; proteinase inhibitor |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 26 Jul 2016 14:56 |
Last Modified: | 23 Jun 2023 22:10 |
Published Version: | http://dx.doi.org/10.3389/fpls.2016.01122 |
Status: | Published |
Publisher: | Frontiers Media |
Identification Number: | 10.3389/fpls.2016.01122 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:102895 |