Gonzalez-Bellido, P.T., Wardill, T.J., Kostyleva, R. et al. (2 more authors) (2009) Overexpressing temperature-sensitive dynamin decelerates phototransduction and bundles microtubules in drosophila photoreceptors. Journal of Neuroscience, 29 (45). pp. 14199-14210. ISSN 0270-6474
Abstract
shibire(ts1), a temperature-sensitive mutation of the Drosophila gene encoding a Dynamin orthologue, blocks vesicle endocytosis and thus synaptic transmission, at elevated, or restrictive temperatures. By targeted Gal4 expression, UAS-shibire(ts1) has been used to dissect neuronal circuits. We investigated the effects of UAS-shibire(ts1) overexpression in Drosophila photoreceptors at permissive (19 degrees C) and restrictive (31 degrees C) temperatures. At 19 degrees C, overexpression of UAS-shi(ts1) causes decelerated phototransduction and reduced neurotransmitter release. This phenotype is exacerbated with dark adaptation, age and in white mutants. Photoreceptors overexpressing UAS-shibire(ts1) contain terminals with widespread vacuolated mitochondria, reduced numbers of vesicles and bundled microtubules. Immuno-electron microscopy reveals that the latter are dynamin coated. Further, the microtubule phenotype is not restricted to photoreceptors, as UAS-shibire(ts1) overexpression in lamina cells also bundles microtubules. We conclude that dynamin has multiple functions that are interrupted by UAS-shibire(ts1) overexpression in Drosophila photoreceptors, destabilizing their neural communication irreversibly at previously reported permissive temperatures.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2009 Society for Neuroscience. Reproduced in accordance with the publisher's self-archiving policy. |
Keywords: | Long-term Adaption; Subcellular Translocation; Rhodopsin Endocytosis; TRPL Channels; Melanogaster; Shibire; Mutant; Histamine; Protein; Cell |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Depositing User: | Miss Anthea Tucker |
Date Deposited: | 02 Dec 2009 16:07 |
Last Modified: | 07 Jun 2014 08:41 |
Published Version: | http://dx.doi.org/10.1523/JNEUROSCI.2873-09.2009 |
Status: | Published |
Publisher: | Society for Neuroscience |
Refereed: | Yes |
Identification Number: | 10.1523/JNEUROSCI.2873-09.2009 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:10223 |