Klepsch, MM, Schmitt, M, Knox, JP orcid.org/0000-0002-9231-6891 et al. (1 more author) (2016) The chemical identity of intervessel pit membranes in Acer challenges hydrogel control of xylem hydraulic conductivity. AoB PLANTS, 8. plw052. ISSN 2041-2851
Abstract
Ion-mediated enhancement of the hydraulic conductivity of xylem tissue (i.e., the ionic effect) has been reported for various angiosperm species. One explanation of the ionic effect is that it is caused by the swelling and shrinking of intervessel pit membranes due to the presence of pectins and/or other cell wall matrix polymers such as heteroxylans or arabinogalactan-proteins (AGPs) that may contain acidic sugars. Here, we examined the ionic effect for six Acer species and their pit membrane chemistry using immunocytochemistry, including antibodies against glycoproteins. Moreover, anatomical features related to the bordered pit morphology and vessel dimensions were investigated using light and electron microscopy. The ionic effect varied from 18% (± 9) to 32% (± 13). Epitopes of homogalacturonan (LM18) and xylan (LM11) were not detected in intervessel pit membranes. Negative results were also obtained for glycoproteins (extensin: LM1, JIM20; AGP glycan: LM2), although AGP (JIM13) related epitopes were detected in parenchyma cells. The mean vessel length was significantly correlated with the magnitude of the ionic effect, unlike other pit or vessel related characteristics.Our results suggest that intervessel pit membranes of Acer are unlikely to contain pectic or other acidic polysaccharides. Therefore, alternative explanations should be tested to clarify the ionic effect.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016, The Authors. Published by Oxford University Press on behalf of the Annals of Botany Company. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Acer; glycoproteins; hydraulic conductivity; immunocytochemistry; ionic effect; pectic polysaccharides; pit membrane; vessel |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 11 Jul 2016 10:01 |
Last Modified: | 05 Oct 2017 16:19 |
Published Version: | http://dx.doi.org/10.1093/aobpla/plw052 |
Status: | Published |
Publisher: | Oxford University Press |
Identification Number: | 10.1093/aobpla/plw052 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:102013 |