Corns, L.F. orcid.org/0000-0003-1139-3527 and Marcotti, W. orcid.org/0000-0002-8770-7628 (2016) Piezo1 haploinsufficiency does not alter mechanotransduction in mouse cochlear outer hair cells. Physiological Reports, 4 (3). e12701-e12701. ISSN 2051-817X
Abstract
The mechanoelectrical transducer (MET) channels located at the stereocilia tip of cochlear hair cells are crucial to convert the mechanical energy of sound into receptor potentials, but the identity of its pore‐forming subunits remains uncertain. Piezo1, which has been identified in the transcriptome of mammalian cochlear hair cells, encodes a transmembrane protein that forms mechanosensitive channels in other tissues. We investigated the properties of the MET channel in outer hair cells (OHCs) of Piezo1 mice (postnatal day 6–9). The MET current was elicited by deflecting the hair bundle of OHCs using sinewave and step stimuli from a piezo‐driven fluid jet. Apical and basal OHCs were investigated because the properties of the MET channel vary along the cochlea. We found that the maximal MET current amplitude and the resting open probability of the MET channel in OHCs were similar between Piezo1+/− haploinsufficient mice and wild‐type littermates. The sensitivity to block by the permeant MET channel blocker dihydrostreptomycin was also similar between the two genotypes. Finally, the anomalous mechano‐gated current, which is activated by sheer force and which is tip‐link independent, was unaffected in OHCs from Piezo1+/− haploinsufficient mice. Our results suggest that Piezo1 is unlikely to be a component of the MET channel complex in mammalian cochlear OHCs
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Funding Information: | Funder Grant number WELLCOME TRUST (THE) 102892/Z/13/Z |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 14 Jul 2016 13:02 |
Last Modified: | 14 Jul 2016 13:02 |
Published Version: | http://dx.doi.org/10.14814/phy2.12701 |
Status: | Published |
Publisher: | Wiley Open Access |
Refereed: | Yes |
Identification Number: | 10.14814/phy2.12701 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:101922 |