Gul, S.S., Douglas, C.W.I., Griffiths, G.S. et al. (1 more author) (2016) A pilot study of active enzyme levels in gingival crevicular fluid of patients with chronic periodontal disease. Journal of Clinical Periodontology, 43 (8). pp. 629-636. ISSN 0303-6979
Abstract
Aim: To determine whether combinations of enzymes in gingival crevicular fluid (GCF) can act as improved biomarkers compared with single enzymes for predicting the outcome of treatment and also for diagnosing the clinical status of sites.
Methods: Thirty subjects with chronic periodontitis were recruited to a 12-month longitudinal pilot study. GCF samples from three representative sites: healthy (≤3 mm), deep non-bleeding (NB) (≥6 mm) and deep bleeding (DB) (≥6 mm) sites and clinical data were collected at baseline, 3 months, 6 months and 12 months following periodontal treatment. Active enzyme levels (MMP-8, cathepsin G, elastase, trypsin-like activity and sialidase) in GCF samples were assessed. The enzyme profiles and clinical data of each site were analysed for correlation and logistic regression was performed to find the predictive value of the active enzyme levels regarding the outcome of treatment.
Results: Twenty-two individuals completed the study. All active enzyme levels were significantly higher in diseased sites than healthy sites. Logistic regression showed that the combination of MMP8, elastase and sialidase provided accurate predictions of treatment outcome (88% for NB and 86% for DB), which was significantly better than each enzyme alone (61%).
Conclusion: This pilot has suggested that combined active enzyme profiling could provide significant prediction of outcome of treatment.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. This is an author produced version of a paper subsequently published in Journal of Clinical Periodontology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | biomarkers; gingival crevicular fluid; longitudinal; outcome of treatment; periodontal disease |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Clinical Dentistry (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 08 Jul 2016 09:17 |
Last Modified: | 18 Jul 2017 05:44 |
Published Version: | http://dx.doi.org/10.1111/jcpe.12568 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1111/jcpe.12568 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:101877 |