Hughes, ML and Dougan, L (2016) The physics of pulling polyproteins: a review of single molecule force spectroscopy using the AFM to study protein unfolding. Reports on Progress in Physics, 79 (7). 076601. ISSN 0034-4885
Abstract
One of the most exciting developments in the field of biological physics in recent years is the ability to manipulate single molecules and probe their properties and function. Since its emergence over two decades ago, single molecule force spectroscopy has become a powerful tool to explore the response of biological molecules, including proteins, DNA, RNA and their complexes, to the application of an applied force. The force versus extension response of molecules can provide valuable insight into its mechanical stability, as well as details of the underlying energy landscape. In this review we will introduce the technique of single molecule force spectroscopy using the atomic force microscope (AFM), with particular focus on its application to study proteins. We will review the models which have been developed and employed to extract information from single molecule force spectroscopy experiments. Finally, we will end with a discussion of future directions in this field.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016, IOP Publishing Ltd. This is an author-created, un-copyedited version of an article published in Reports on Progress in Physics. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at http://dx.doi.org/10.1088/0034-4885/79/7/076601. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | SMFS, biophysics, polyprotein, force, thermodynamic, kinetic, biological physics |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Molecular & Nanoscale Physics |
Depositing User: | Symplectic Publications |
Date Deposited: | 29 Jun 2016 13:13 |
Last Modified: | 18 Jul 2017 05:15 |
Published Version: | http://dx.doi.org/10.1088/0034-4885/79/7/076601 |
Status: | Published |
Publisher: | IOP Publishing |
Identification Number: | 10.1088/0034-4885/79/7/076601 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:101530 |