Jaafar Marican, H., Cruz-Migoni, S. and Borycki, A.G. (2016) Asymmetric distribution of primary cilia allocates satellite cells for self-renewal. Stem Cell Reports, 6 (6). pp. 798-805.
Abstract
Regeneration of vertebrate skeletal muscles requires satellite cells, a population of stem cells that are quiescent in normal conditions and divide, differentiate, and self-renew upon activation triggered by exercise, injury, and degenerative diseases. Satellite cell self-renewal is essential for long-term tissue homeostasis, and previous work has identified a number of external cues that control this process. However, little is known of the possible intrinsic control mechanisms of satellite cell self-renewal. Here, we show that quiescent satellite cells harbor a primary cilium, which is rapidly disassembled upon entry into the cell cycle. Contrasting with a commonly accepted belief, cilia reassembly does not occur uniformly in cells exiting the cell cycle. We found that primary cilia reassemble preferentially in cells committed to self-renew, and disruption of cilia reassembly causes a specific deficit in self-renewing satellite cells. These observations indicate that primary cilia provide an intrinsic cue essential for satellite cell self-renewal.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) |
Funding Information: | Funder Grant number ASSOCIATION FRANCAISE CONTRE LES MYOPATHIES 16793 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 05 Jul 2016 10:07 |
Last Modified: | 06 Nov 2018 13:50 |
Published Version: | http://dx.doi.org/10.1016/j.stemcr.2016.04.004 |
Status: | Published |
Publisher: | Elsevier |
Refereed: | Yes |
Identification Number: | 10.1016/j.stemcr.2016.04.004 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:101527 |