Dew, L., English, W.R. orcid.org/0000-0003-3024-2441, Ortega, I. et al. (2 more authors) (2016) Fabrication of biodegradable synthetic vascular networks and their use as a model of angiogenesis. Cells Tissues Organs. 446644. ISSN 1422-6405
Abstract
One of the greatest challenges currently faced in tissue engineering is the incorporation of vascular networks within tissue-engineered constructs. The aim of this study was to develop a technique for producing a perfusable, three-dimensional cell friendly model of vascular structures that could be used to study the factors affecting angiogenesis and vascular biology in engineered systems in more detail. Initially, biodegradable synthetic pseudo-vascular networks were produced via the combination of robocasting and electrospinning techniques. The internal surfaces of the vascular channels were then recellularized with human dermal microvascular endothelial cells (HDMECs) with and without the presence of human dermal fibroblasts (HDFs) on the outer surface of the scaffold. After 7 days in culture, channels that had been reseeded with HDMECs alone, demonstrated irregular cell coverage. However when using a co-culture of HDMECs inside and HDFs outside the vascular channels, coverage was found to be continuous throughout the internal channel. Using this cell combination, collagen gels loaded with vascular endothelial growth factor were deposited onto the outer surface of the scaffold and cultured for a further 7 days after which endothelial cell (EC) outgrowth from within the channels into the collagen gel was observed showing the engineered vasculature maintains its capacity for angiogenesis. Furthermore the HDMECs appeared to have formed perfusable tubules within the gel. These results show promising steps towards the development of an in vitro platform upon which to study angiogenesis and vascular biology in a tissue-engineering context.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2016 S. Karger AG, Basel. This is an author produced version of a paper subsequently published in Cells Tissues Organs. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Angiogenesis; Electrospinning; Robocasting; Tissue Engineering; Regenerative Medicine |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) The University of Sheffield > Sheffield Teaching Hospitals |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 27 Jun 2016 09:14 |
Last Modified: | 06 Sep 2017 11:43 |
Published Version: | https://dx.doi.org/10.1159/000446644 |
Status: | Published |
Publisher: | Karger |
Refereed: | Yes |
Identification Number: | 10.1159/000446644 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:101405 |