Janganan, T.K., Mullin, N., Tzokov, S.B. et al. (5 more authors) (2016) Characterization of the spore surface and exosporium proteins of Clostridium sporogenes; implications for C. botulinum Group I strains. Food Microbiology, 59. pp. 205-212. ISSN 0740-0020
Abstract
Clostridium sporogenes is a non-pathogenic close relative and surrogate for Group I (proteolytic) neurotoxin-producing Clostridium botulinum strains. The exosporium, the sac-like outermost layer of spores of these species, is likely to contribute to adhesion, dissemination, and virulence. A paracrystalline array, hairy nap, and several appendages were detected in the exosporium of C. sporogenes strain NCIMB 701792 by EM and AFM. The protein composition of purified exosporium was explored by LC-MS/MS of tryptic peptides from major individual SDS-PAGE-separated protein bands, and from bulk exosporium. Two high molecular weight protein bands both contained the same protein with a collagen-like repeat domain, the probable constituent of the hairy nap, as well as cysteine-rich proteins CsxA and CsxB. A third cysteine-rich protein (CsxC) was also identified. These three proteins are also encoded in C. botulinum Prevot 594, and homologues (75–100% amino acid identity) are encoded in many other Group I strains. This work provides the first insight into the likely composition and organization of the exosporium of Group I C. botulinum spores.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Keywords: | Spore appendages; Two dimensional crystals; Cysteine rich proteins |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Funding Information: | Funder Grant number BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL (BBSRC) BB/G004323/1 ROYAL SOCIETY TG092324 WELLCOME TRUST (THE) 091322/B/Z/10/Z |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 23 Jun 2016 10:09 |
Last Modified: | 06 Jun 2017 00:38 |
Published Version: | https://dx.doi.org/10.1016/j.fm.2016.06.003 |
Status: | Published |
Publisher: | Elsevier |
Refereed: | Yes |
Identification Number: | 10.1016/j.fm.2016.06.003 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:101259 |