Kapoore, R.V. orcid.org/0000-0002-2287-0619 and Vaidyanathan, S. orcid.org/0000-0003-4137-1230 (2016) Towards quantitative mass spectrometry based metabolomics in microbial and mammalian systems. Philosophical transactions of the Royal Society of London. Series A: Mathematical and physical sciences, 374. 20150363. ISSN 0080-4614
Abstract
Metabolome analyses are a suite of analytical approaches that enable us to capture changes in the metabolome (small molecular weight components, typically less than 1500 Da) in biological systems. Mass spectrometry (MS) has been widely used for this purpose. The key challenge here is to be able to capture changes in a reproducible and reliant manner that is representative of the events that take place in vivo. Typically, the analysis is carried out in vitro, by isolating the system and extracting the metabolome. MS-based approaches enable us to capture metabolomic changes with high sensitivity and resolution. When developing the technique for different biological systems, there are similarities in challenges and differences that are specific to the system under investigation. Here, we review some of the challenges in capturing quantitative changes in the metabolome with MS based approaches, primarily in microbial and mammalian systems.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 The Author(s) Published by the Royal Society. This is an author produced version of a paper subsequently published in Philosophical transactions of the Royal Society of London. Series A: Mathematical and physical sciences . Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield) |
Funding Information: | Funder Grant number BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL (BBSRC) BB/K020633/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 26 Sep 2016 09:53 |
Last Modified: | 20 Sep 2017 04:12 |
Published Version: | http://dx.doi.org/10.1098/rsta.2015.0363 |
Status: | Published |
Publisher: | Royal Society, The |
Refereed: | Yes |
Identification Number: | 10.1098/rsta.2015.0363 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:101246 |