Actis, P orcid.org/0000-0002-7146-1854, Maalouf, MM, Kim, HJ et al. (4 more authors) (2014) Compartmental Genomics in Living Cells Revealed by Single-Cell Nanobiopsy. ACS Nano, 8 (1). pp. 546-553. ISSN 1936-0851
Abstract
The ability to study the molecular biology of living single cells in heterogeneous cell populations is essential for next generation analysis of cellular circuitry and function. Here, we developed a single-cell nanobiopsy platform based on scanning ion conductance microscopy (SICM) for continuous sampling of intracellular content from individual cells. The nanobiopsy platform uses electrowetting within a nanopipette to extract cellular material from living cells with minimal disruption of the cellular milieu. We demonstrate the subcellular resolution of the nanobiopsy platform by isolating small subpopulations of mitochondria from single living cells, and quantify mutant mitochondrial genomes in those single cells with high throughput sequencing technology. These findings may provide the foundation for dynamic subcellular genomic analysis.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/nn405097u. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | electrowetting; mitochondria; nanopipette; next-generation sequencing; scanning ion conductance microscopy; single-cell biopsy |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) > Pollard Institute (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 24 Jun 2016 14:32 |
Last Modified: | 25 Sep 2020 16:32 |
Published Version: | http://dx.doi.org/10.1021/nn405097u |
Status: | Published |
Publisher: | American Chemical Society |
Identification Number: | 10.1021/nn405097u |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:101074 |