Actis, P orcid.org/0000-0002-7146-1854, Tokar, S, Clausmeyer, J et al. (18 more authors) (2014) Electrochemical Nanoprobes for Single-Cell Analysis. ACS Nano, 8 (1). pp. 875-884. ISSN 1936-0851
Abstract
The measurement of key molecules in individual cells with minimal disruption to the biological milieu is the next frontier in single-cell analyses. Nanoscale devices are ideal analytical tools because of their small size and their potential for high spatial and temporal resolution recordings. Here, we report the fabrication of disk-shaped carbon nanoelectrodes whose radius can be precisely tuned within the range 5–200 nm. The functionalization of the nanoelectrode with platinum allowed the monitoring of oxygen consumption outside and inside a brain slice. Furthermore, we show that nanoelectrodes of this type can be used to impale individual cells to perform electrochemical measurements within the cell with minimal disruption to cell function. These nanoelectrodes can be fabricated combined with scanning ion conductance microscopy probes, which should allow high resolution electrochemical mapping of species on or in living cells.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/nn405612q. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | carbon; nanoelectrode; platinum; electrochemistry; intracellular measurements; brain slice; melanoma |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) > Pollard Institute (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 24 Jun 2016 14:45 |
Last Modified: | 27 Oct 2020 16:30 |
Published Version: | http://dx.doi.org/10.1021/nn405612q |
Status: | Published |
Publisher: | American Chemical Society |
Identification Number: | 10.1021/nn405612q |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:101073 |