Ivanov, AP, Actis, P orcid.org/0000-0002-7146-1854, Jonsson, P et al. (3 more authors) (2015) On-Demand Delivery of Single DNA Molecules Using Nanopipets. ACS Nano, 9 (4). pp. 3587-3595. ISSN 1936-0851
Abstract
Understanding the behavioral properties of single molecules or larger scale populations interacting with single molecules is currently a hotly pursued topic in nanotechnology. This arises from the potential such techniques have in relation to applications such as targeted drug delivery, early stage detection of disease, and drug screening. Although label and label-free single molecule detection strategies have existed for a number of years, currently lacking are efficient methods for the controllable delivery of single molecules in aqueous environments. In this article we show both experimentally and from simulations that nanopipets in conjunction with asymmetric voltage pulses can be used for label-free detection and delivery of single molecules through the tip of a nanopipet with “on-demand” timing resolution. This was demonstrated by controllable delivery of 5 kbp and 10 kbp DNA molecules from solutions with concentrations as low as 3 pM.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2015, American Chemical Society. This is an author produced version of a paper published in ACS Nano. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | single-molecule delivery; label-free detection; nanopore; nanopipet; DNA |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) > Pollard Institute (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 21 Jun 2016 10:34 |
Last Modified: | 17 Feb 2019 05:04 |
Published Version: | http://dx.doi.org/10.1021/acsnano.5b00911 |
Status: | Published |
Publisher: | American Chemical Society |
Identification Number: | 10.1021/acsnano.5b00911 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:101070 |