Brown, S.P., Cornell, S.J., Sheppard, M. et al. (4 more authors) (2006) Intracellular Demography and the Dynamics of Salmonella enterica Infections. PLoS Biology, 4 (11). e349. ISSN 1544-9173
Abstract
An understanding of within-host dynamics of pathogen interactions with eukaryotic cells can shape the development of effective preventive measures and drug regimes. Such investigations have been hampered by the difficulty of identifying and observing directly, within live tissues, the multiple key variables that underlay infection processes. Fluorescence microscopy data on intracellular distributions of Salmonella enterica serovar Typhimurium (S. Typhimurium) show that, while the number of infected cells increases with time, the distribution of bacteria between cells is stationary (though highly skewed). Here, we report a simple model framework for the intensity of intracellular infection that links the quasi-stationary distribution of bacteria to bacterial and cellular demography. This enables us to reject the hypothesis that the skewed distribution is generated by intrinsic cellular heterogeneities, and to derive specific predictions on the within-cell dynamics of Salmonella division and host-cell lysis. For within-cell pathogens in general, we show that within-cell dynamics have implications across pathogen dynamics, evolution, and control, and we develop novel generic guidelines for the design of antibacterial combination therapies and the management of antibiotic resistance.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2006 Brown et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > Institute of Integrative and Comparative Biology (Leeds) |
Depositing User: | Sherpa Assistant |
Date Deposited: | 06 Nov 2009 12:26 |
Last Modified: | 04 Nov 2016 04:01 |
Published Version: | http://dx.doi.org/10.1371/journal.pbio.0040349 |
Status: | Published |
Publisher: | Public Library of Science |
Identification Number: | doi: 10.1371/journal.pbio.0040349 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:10102 |