Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase

Abstract

Lytic polysaccharide monooxygenases (LPMOs) are recently discovered enzymes that oxidatively deconstruct polysaccharides. LPMOs are fundamental in the effective utilization of these substrates by bacteria and fungi; moreover, the enzymes have significant industrial importance. We report here the activity, spectroscopy and three-dimensional structure of a starch-active LPMO, a representative of the new CAZy AA13 family. We demonstrate that these enzymes generate aldonic acid-terminated malto-oligosaccharides from retrograded starch and boost significantly the conversion of this recalcitrant substrate to maltose by β-amylase. The detailed structure of the enzyme’s active site yields insights into the mechanism of action of this important class of enzymes.

Metadata

Item Type:	Article
Authors/Creators:	Lo Leggio, L Simmons, TJ Poulsen, J-CN Frandsen, KEH Hemsworth, GR https://orcid.org/0000-0002-8226-1380 Stringer, MA Von Freiesleben, P Tovborg, M Johansen, KS De Maria, L Harris, PV Soong, C-L Dupree, P Tryfona, T Lenfant, N Henrissat, B Davies, GJ Walton, PH
Copyright, Publisher and Additional Information:	© 2015 Macmillan Publishers Limited. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Keywords:	Structural biology; Biotechnology
Dates:	Published: 22 January 2015 Accepted: 25 November 2014
Institution:	The University of Leeds
Academic Units:	The University of Leeds > Faculty of Biological Sciences (Leeds)
Depositing User:	Symplectic Publications
Date Deposited:	07 Oct 2016 08:59
Last Modified:	07 Oct 2016 08:59
Published Version:	http://dx.doi.org/10.1038/ncomms6961
Status:	Published
Publisher:	Nature Publishing Group
Identification Number:	10.1038/ncomms6961
Open Archives Initiative ID (OAI ID):	oai:eprints.whiterose.ac.uk:100906

CORE (COnnecting REpositories)

Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase

Abstract

Metadata

Download

Published Version

Export

Statistics