Tse, C., Whiteley, R., Yu, T. et al. (4 more authors) (2016) Inkjet printing Schwann cells and neuronal analogue NG108-15 cells. Biofabrication, 8 (1). 015017. ISSN 1758-5082
Abstract
Porcine Schwann cells and neuronal analogue NG108-15 cells were printed using a piezoelectric-inkjet-printer with a nozzle diameter of 60 μm, within the range of 70–230 V, with analysis of viability and quality after printing. Neuronal and glial cell viabilities of >86% and >90% were detected immediately after printing and no correlation between voltage applied and cell viability could be seen. Printed neuronal cells were shown to produce neurites earlier compared to controls, and over several days, produced longer neurites which become most evident by day 7. The number of neurites becomes similar by day 7 also, and cells proliferate with a similar viability to that of non-printed cells (controls). This method of inkjet printing cells provides a technical platform for investigating neuron–glial cell interactions with no significant difference to cell viability than standard cell seeding. Such techniques can be utilized for lab-on-a-chip technologies and to create printed neural networks for neuroscience applications.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 IOP Publishing Ltd. This is an author produced version of a paper subsequently published in Biofabrication. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | inkjet printing; cell printing; nerve printing; tissue engineering |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Mechanical Engineering (Sheffield) |
Funding Information: | Funder Grant number US OFFICE OF NAVAL RESEARCH W911NF-14-1-0581 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 31 May 2016 09:07 |
Last Modified: | 12 Apr 2017 20:38 |
Published Version: | http://dx.doi.org/10.1088/1758-5090/8/1/015017 |
Status: | Published |
Publisher: | IOP Publishing |
Refereed: | Yes |
Identification Number: | 10.1088/1758-5090/8/1/015017 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:100271 |