Song, L, Guo, Y orcid.org/0000-0003-4607-7356, Roebuck, D et al. (10 more authors) (2015) Terminal PEGylated DNA-Gold Nanoparticle Conjugates Offering High Resistance to Nuclease Degradation and Efficient Intracellular Delivery of DNA Binding Agents. ACS Applied Materials and Interfaces, 7 (33). pp. 18707-18716. ISSN 1944-8244
Abstract
Over the past 10 years, polyvalent DNA-gold nanoparticle (DNA-GNP) conjugate has been demonstrated as an efficient, universal nanocarrier for drug and gene delivery with high uptake by over 50 different types of primary and cancer cell lines. A barrier limiting its in vivo effectiveness is limited resistance to nuclease degradation and non-specific interaction with blood serum contents. Herein we show that terminal PEGylation of the complementary DNA strand hybridized to a polyvalent DNA-GNP conjugate can eliminate non-specific adsorption of serum proteins and greatly increases its resistance against DNase I based degradation. The PEGylated DNA-GNP conjugate still retains high cell uptake property, making it an attractive intracellular delivery nanocarrier for DNA binding reagents. We show it can be used for successful intracellular delivery of doxorubicin, a widely used clinical cancer chemotherapeutic drug. Moreover, it can be used for efficient delivery of some cell-membrane impermeable reagents such as propidium iodide (a DNA intercalating fluorescent dye currently limited to the use of staining dead cells only) and a di-ruthenium complex (a DNA groove binder), for successful staining of live cells.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials and Interfaces, © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acsami.5b05228 |
Keywords: | GNP-DNA conjugate; enzymatic digestion; drug delivery; PEGylation; bioimaging; DNA binding agent |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Inorganic Chemistry (Leeds) The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Chemistry and Biochemistry (Leeds) |
Funding Information: | Funder Grant number Wellcome Trust 097354/Z/11/Z The Wellcome Trust UNSPECIFIED EU - European Union 331281 EU - European Union 331281 EU - European Union 331281 |
Depositing User: | Symplectic Publications |
Date Deposited: | 10 Aug 2015 12:31 |
Last Modified: | 26 Oct 2020 18:01 |
Published Version: | http://dx.doi.org/10.1021/acsami.5b05228 |
Status: | Published |
Publisher: | American Chemical Society |
Identification Number: | 10.1021/acsami.5b05228 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:88760 |