Serrano, CA, Villagran, A, Toledo, H et al. (2 more authors) (2016) Iron deficiency and IL1β polymorphisms in Helicobacter pylori-infected children. Helicobacter, 21 (2). pp. 124-130. ISSN 1083-4389
Abstract
Background: Helicobacter pylori-infection has been associated with an imbalance of iron homeostasis. IL-1 has been related with iron absorption disturbances through a variety of mechanisms. The aim of this study was to evaluate the presence of polymorphic variants for IL-1 cluster and gastric IL1β mRNA expression in H. pylori-infected children and their relationship with hypoclorhydria and iron deficiency (ID). Patients and Methods: Prospective study of 123 symptomatic children. At endoscopy, antral biopsies were taken for urease test, pathology and culture and blood for analysis of ferritin, transferrin, serum iron and total iron-binding capacity. Polymorphisms in the IL-1 cluster (positions -511, -31, +3954, ILRN) were determined by PCR-RFLP. Gastric mucosal expression of IL-1 mRNA was determined by RT-PCR. Results: After exclusions, out of 105 patients, 33 (31.4%) were H. pylori positive. Nine (8.6%) children were classified as iron deficient (ID). H. pylori positivity was associated with ID (OR: 5.1; 95% CI: 1.2-21.9) (p = 0.04). No significant differences were found in allele frequency for IL1β gene cluster polymorphisms between infected and uninfected children. H. pylori-infected children with ID had significantly increased gastric IL1β mRNA in comparison to infected children without ID. In addition a significant positive correlation was observed between mucosal IL-1β mRNA and fasting gastric juice pH. Gastric pH values were significantly increased in H. pylori infected patients with ID compared to uninfected children. Conclusions: The established association between H. pylori infection and ID in children may be mediated by increased gastric mucosal IL-1.
Metadata
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Copyright, Publisher and Additional Information: | © 2015, Wiley. This is the peer reviewed version of the following article: “Serrano, CA, Villagran, A, Toledo, H, Crabtree, JE and Harris, PR (2015) Iron deficiency and IL1β polymorphisms in Helicobacter pylori-infected children. Helicobacter”, which has been published in final form at http://dx.doi.org/10.1111/hel.12247. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | ||||
Keywords: | H. pylori, children, iron deficiency, Interleukin-1 polymorphisms | ||||
Dates: |
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Institution: | The University of Leeds | ||||
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds) > Section of Molecular Gastroenterology (Leeds) | ||||
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Depositing User: | Symplectic Publications | ||||
Date Deposited: | 30 Jul 2015 10:44 | ||||
Last Modified: | 29 Oct 2016 07:30 | ||||
Published Version: | http://dx.doi.org/10.1111/hel.12247 | ||||
Status: | Published | ||||
Publisher: | Wiley | ||||
Identification Number: | https://doi.org/10.1111/hel.12247 |