Williams, D.M. orcid.org/0000-0001-8483-021X, Hornsby, H.R. orcid.org/0000-0002-6657-9743, Shehata, O.M. et al. (19 more authors) (2023) Establishing SARS-CoV-2 membrane protein-specific antibodies as a valuable serological target via high-content microscopy. iScience, 26 (7). 107056. ISSN 2589-0042
Abstract
The prevalence and strength of serological responses mounted toward SARS-CoV-2 proteins other than nucleocapsid (N) and spike (S), which may be of use as additional serological markers, remains underexplored. Using high-content microscopy to assess antibody responses against full-length StrepTagged SARS-CoV-2 proteins, we found that 85% (166/196) of unvaccinated individuals with RT-PCR confirmed SARS-CoV-2 infections and 74% (31/42) of individuals infected after being vaccinated developed detectable IgG against the structural protein M, which is higher than previous estimates. Compared with N antibodies, M IgG displayed a shallower time-dependent decay and greater specificity. Sensitivity for SARS-CoV-2 seroprevalence was enhanced when N and M IgG detection was combined. These findings indicate that screening for M seroconversion may be a good approach for detecting additional vaccine breakthrough infections and highlight the potential to use HCM as a rapidly deployable method to identify the most immunogenic targets of newly emergent pathogens.
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Copyright, Publisher and Additional Information: | © 2023 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | ||||||||||||||
Keywords: | Biological sciences; Cell biology; Immunology; Microbiology | ||||||||||||||
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Institution: | The University of Sheffield | ||||||||||||||
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Medicine and Population Health The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Infection, Immunity and Cardiovascular Disease |
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Depositing User: | Symplectic Sheffield | ||||||||||||||
Date Deposited: | 09 Jan 2024 08:52 | ||||||||||||||
Last Modified: | 09 Jan 2024 08:52 | ||||||||||||||
Status: | Published | ||||||||||||||
Publisher: | Elsevier BV | ||||||||||||||
Refereed: | Yes | ||||||||||||||
Identification Number: | https://doi.org/10.1016/j.isci.2023.107056 | ||||||||||||||
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