Bioavailability and excretion profile of betacyanins – Variability and correlations between different excretion routes

The present study addresses the knowledge gap in betalain bioavailability, transformation and excretion. Analysis of renal and fecal excretion profiles in humans after consumption of beetroot revealed very low bioavailability (renal recovery of 0.13 %) and fast elimination of pigments (renal elimination rate constant of 0.16 h−1), while the majority of betalains underwent severe depletion during GI transit, evidenced by decarboxylation, deglucosidation and dehydrogenation. Betacyanin metabolite levels in human urine were positively associated with those in stools (p < 0.05), indicating significant impact of pigment metabolism in the gut on their bioavailability. In addition, the current study revealed large inter-individual and compositional variabilities of pigment after colonic fermentation compared with systemic metabolism, likely attributed to the increasing complexity of intestinal environment with diverse gut microbiota. To conclude, intestinal uptake and systemic metabolism of betacyanins are intimately associated with their intestinal biotransformation, with gut microbiota serving as a crucial factor.