Hammond, F.R., Lewis, A., Speirs, Z.C. et al. (7 more authors) (2023) An arginase 2 promoter transgenic illuminates immune cell polarisation in zebrafish. Disease Models & Mechanisms, 16 (6). dmm049966. ISSN 1754-8403
Abstract
Innate immune responses to inflammation and infection are complex and represent major challenges for developing much needed new treatments for chronic inflammatory diseases and drug-resistant infections. To be ultimately successful, the immune response must be balanced to allow pathogen clearance without excess tissue damage, processes controlled by pro- and anti-inflammatory signals. The roles of anti-inflammatory signalling in raising an appropriate immune response are underappreciated, representing overlooked potential drug targets. This is especially true in neutrophils, a difficult cell type to study ex vivo owing to a short lifespan, dogmatically seen as being highly pro-inflammatory. Here, we have generated and describe the first zebrafish transgenic line [TgBAC(arg2:eGFP)sh571] that labels expression of the anti-inflammatory gene arginase 2 (arg2) and show that a subpopulation of neutrophils upregulate arginase soon after immune challenge with injury and infection. At wound-healing stages, arg2:GFP is expressed in subsets of neutrophils and macrophages, potentially representing anti-inflammatory, polarised immune cell populations. Our findings identify nuanced responses to immune challenge in vivo, responses that represent new opportunities for therapeutic interventions during inflammation and infection.
Metadata
Authors/Creators: |
|
||||||||
---|---|---|---|---|---|---|---|---|---|
Copyright, Publisher and Additional Information: | © 2023 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | ||||||||
Keywords: | Anti inflammatory; Infection; Inflammation; Macrophage; Neutrophil; Zebrafish | ||||||||
Dates: |
|
||||||||
Institution: | The University of Sheffield | ||||||||
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Infection, Immunity and Cardiovascular Disease | ||||||||
Funding Information: |
|
||||||||
Depositing User: | Symplectic Sheffield | ||||||||
Date Deposited: | 10 May 2023 14:33 | ||||||||
Last Modified: | 10 May 2023 14:33 | ||||||||
Status: | Published | ||||||||
Publisher: | The Company of Biologists | ||||||||
Refereed: | Yes | ||||||||
Identification Number: | https://doi.org/10.1242/dmm.049966 | ||||||||
Related URLs: |