The impact of viral mutations on recognition by SARS-CoV-2 specific T-cells

This is a preprint and may not have undergone formal peer review

Abstract

We identify amino acid variants within dominant SARS-CoV-2 T-cell epitopes by interrogating global sequence data. Several variants within nucleocapsid and ORF3a epitopes have arisen independently in multiple lineages and result in loss of recognition by epitope-specific T-cells assessed by IFN-γ and cytotoxic killing assays. These data demonstrate the potential for T-cell evasion and highlight the need for ongoing surveillance for variants capable of escaping T-cell as well as humoral immunity.

Metadata

Authors/Creators:

de Silva, T.I. https://orcid.org/0000-0002-6498-9212
Liu, G.
B Lindsey, B.
Dong, D.
Shah, D.
J. Mentzer, A.
Angyal, A.
Brown, R.
D. Parker, M. https://orcid.org/0000-0003-2999-3870
Ying, Z.
Yao, X.
Turtle, L.
Dunachie, S.
K. Maini, M.
Ogg, G.
Knight, J.C.
Peng, Y.
Rowland-Jones, S.L. https://orcid.org/0000-0001-5788-6998
Dong, T.

Copyright, Publisher and Additional Information:

© 2021 The Author(s). This preprint is made available under a Creative Commons Attribution 4.0 International License. (https://creativecommons.org/licenses/by/4.0/)

Keywords:

COVID-19 Genomics UK (COG-UK) Consortium

Dates:

Submitted: 8 April 2021

Institution:

The University of Sheffield

Academic Units:

The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Infection, Immunity and Cardiovascular Disease

Funding Information:

Funder	Grant number
National Institute for Health Research	IS-BRC-1215-20017
WELLCOME TRUST (THE)	110058/A/15/Z
MEDICAL RESEARCH COUNCIL	MR/V028448/1

Depositing User:

Symplectic Sheffield

Date Deposited:

05 May 2023 16:04

Last Modified:

05 May 2023 16:04

Published Version:

http://dx.doi.org/10.1101/2021.04.08.438904

Status:

Submitted

Publisher:

Cold Spring Harbor Laboratory

Identification Number:

https://doi.org/10.1101/2021.04.08.438904

CORE (COnnecting REpositories)

CORE (COnnecting REpositories)