Hume, L, Edge, JC, Tinning, H orcid.org/0000-0002-0840-3205 et al. (12 more authors) (2023) MicroRNAs emerging coordinate with placental mammals alter pathways in endometrial epithelia important for endometrial function. iScience, 26 (4). 106339. ISSN 2589-0042
Abstract
We tested the hypothesis that conserved placental mammal-specific microRNAs and their targets facilitate endometrial receptivity to implantation. Expression of miR-340-5p, -542-3p, and -671-5p was regulated by exposure of endometrial epithelial cells to progesterone (10 mg/ml) for 24 h coordinate with 1,713 of their predicted targets. Proteomic analysis of cells transfected with miRNA mimic/inhibitor (48 h: n = 3) revealed 1,745 proteins altered by miR-340-5p (mimic; 1,369, inhibitor; 376) of which 171 were predicted targets and P4-regulated. MiR-542-3p altered 2,353 (mimic; 1,378, inhibitor; 975) 100 which were mirDB predicted, including 46 P4-regulated. MiR-671-5p altered 1,744 proteins (mimic; 1,252, inhibitor; 492) 95 of which were predicted targets and 46 P4-regulated. All miRNAs were detected in luteal phase endometrial biopsies, irrespective of pregnancy outcomes. miR-340-5p expression increased in biopsies from individuals suffering previous and subsequent miscarriage compared to those with subsequent live birth. Dysfunction of these miRNAs and their targets contribute to endometrial-derived recurrent pregnancy loss.
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Copyright, Publisher and Additional Information: | © 2023 The Author(s). This is an open access article published under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited | ||||
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Institution: | The University of Leeds | ||||
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM) > Discovery & Translational Science Dept (Leeds) | ||||
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Depositing User: | Symplectic Publications | ||||
Date Deposited: | 13 Mar 2023 08:29 | ||||
Last Modified: | 28 Apr 2023 14:55 | ||||
Published Version: | http://dx.doi.org/10.1016/j.isci.2023.106339 | ||||
Status: | Published | ||||
Publisher: | Cell Press | ||||
Identification Number: | https://doi.org/10.1016/j.isci.2023.106339 |
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