Electromagnetic Property Characterization and Sensing of Endothelial Cells Growth Medium and Dulbecco's Phosphate Buffered Saline Solution for in vitro Cell Culture

Presented within this paper are the findings and illustrated experimental results, for the extracting parameters of specific chemical solutions, used to represent the complicated permittivity and the loss tangent of liquids at microwave frequencies. In particular, the endothelial cells growth medium and Dulbecco's Phosphate Buffered Saline (DPBS) solution in the case of the experiment's sample were evaluated in 50 ml water-based solution at a test frequency between 200 MHz and 50 GHz. The vector network analyzer model E8361A was used for measuring complex reflection and permittivity coefficient parameters. From the numerical results, different values of the loss tangent were identified when compared with test samples of plain water in the frequency range of 200 MHz to 10 GHz. Findings obtained produced the same results in the 10 GHz as well as higher frequency ranges. Moreover, the research presents the characteristics, differential measurement results of growth medium used for human endothelial cells and DPBS, two of the most common solutions required for culturing cells in vitro. By enabling to distinguish sample characteristics in response to magnetic resonance, this method has potential to be applied for non-invasive and non-destructive analysis of biological samples in biology and medicine.