Tao, C.-N., Buswell, W., Zhang, P. et al. (4 more authors) (2022) A single amino acid transporter controls the uptake of priming-inducing beta-amino acids and the associated trade-off between induced resistance and plant growth. Plant Cell. koac271. ISSN 1040-4651
Abstract
Selected beta-amino acids, such as beta-aminobutyric acid (BABA) and R-beta-homoserine (RBH), can prime plants for resistance against a broad spectrum of diseases. Here, we describe a genome-wide screen of fully annotated Arabidopsis thaliana T-DNA insertion lines for impaired in RBH-induced immunity (iri) mutants against the downy mildew pathogen Hyaloperonospora arabidopsidis, yielding 104 lines that were partially affected and four lines that were completely impaired in RBH-induced resistance. We confirmed the iri1-1 mutant phenotype with an independent T-DNA insertion line in the same gene, encoding the high-affinity amino acid transporter LYSINE HISTIDINE TRANSPORTER 1 (LHT1). Uptake experiments with yeast cells expressing LHT1 and mass spectrometry-based quantification of RBH and BABA in leaves of lht1 mutant and LHT1 overexpression lines revealed that LHT1 acts as the main transporter for cellular uptake and systemic distribution of RBH and BABA. Subsequent characterization of lht1 mutant and LHT1 overexpression lines for induced resistance and growth responses revealed that the levels of LHT1-mediated uptake determine the trade-off between induced resistance and plant growth by RBH and BABA.
Metadata
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Copyright, Publisher and Additional Information: | © 2022 American Society of Plant Biologists. This is an author-produced version of a paper subsequently published in Plant Cell. Uploaded in accordance with the publisher's self-archiving policy. | ||||||||||||||||||
Keywords: | BABA; RBH; defense priming agents; amino acid transporter; LHT1; 16 Arabidopsis; defense-growth trade-off | ||||||||||||||||||
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Institution: | The University of Sheffield | ||||||||||||||||||
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) | ||||||||||||||||||
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Depositing User: | Symplectic Sheffield | ||||||||||||||||||
Date Deposited: | 09 Sep 2022 10:54 | ||||||||||||||||||
Last Modified: | 30 Aug 2023 00:13 | ||||||||||||||||||
Status: | Published online | ||||||||||||||||||
Publisher: | American Society of Plant Biologists | ||||||||||||||||||
Refereed: | Yes | ||||||||||||||||||
Identification Number: | https://doi.org/10.1093/plcell/koac271 |