Iscaro, A., Jones, C., Forbes, N. et al. (10 more authors) (2022) Targeting circulating monocytes with CCL2-loaded liposomes armed with an oncolytic adenovirus. Nanomedicine: Nanotechnology, Biology and Medicine, 40. 102506. ISSN 1549-9634
Abstract
Oncolytic viruses (OVs) selectively replicate in and destroy cancer cells resulting in anti-tumor immunity. However, clinical use remains a challenge because of virus clearance upon intravenous delivery. OV packaging using a nanomedicine approach could overcome this. Here we encapsulate an oncolytic adenovirus (Ad[I/PPT-E1A]) into CCL2-coated liposomes in order to exploit recruitment of CCR2-expressing circulating monocytes into tumors. We demonstrate successful encapsulation of Ad[I/PPT-E1A] into CCL2-coated liposomes that were preferentially taken up by CCR2-expressing monocytes. No complex-related toxicities were observed following incubation with prostate tumor cells and the encapsulation did not affect virus oncolytic activity in vitro. Furthermore, intravenous administration of our nanomedicine resulted in a significant reduction in tumor size and pulmonary metastasis in prostate cancer-bearing mice whereby a 1000-fold less virus was needed compared to Ad[I/PPT-E1A] alone. Taken together our data provide an opportunity to target OVs via circulation to inaccessible tumors using liposome-assisted drug delivery.
Metadata
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Copyright, Publisher and Additional Information: | © 2021 Elsevier Inc. | ||||||
Keywords: | Adenovirus; Chemokine receptor 2; Liposomes; Monocytes; Oncolytic virus; Prostate cancer; Adenoviridae; Animals; Cell Line, Tumor; Chemokine CCL2; Genetic Vectors; Humans; Liposomes; Male; Mice; Monocytes; Oncolytic Virotherapy | ||||||
Dates: |
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Institution: | The University of Sheffield | ||||||
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) | ||||||
Funding Information: |
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Depositing User: | Symplectic Sheffield | ||||||
Date Deposited: | 11 May 2022 09:40 | ||||||
Last Modified: | 11 May 2022 09:40 | ||||||
Status: | Published | ||||||
Publisher: | Elsevier BV | ||||||
Refereed: | Yes | ||||||
Identification Number: | https://doi.org/10.1016/j.nano.2021.102506 | ||||||
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