Fessl, T, Crossley, JA, Watkins, D et al. (6 more authors) (2021) Dynamics of Membrane Proteins Monitored by Single-Molecule Fluorescence Across Multiple Timescales. In: Postis, VLG and Goldman, A, (eds.) Biophysics of Membrane Proteins: Methods and Protocols. Methods in Molecular Biology, 2168 . Humana , New York, NY , pp. 273-297. ISBN 978-1-0716-0723-7
Abstract
Single-molecule techniques provide insights into the heterogeneity and dynamics of ensembles and enable the extraction of mechanistic information that is complementary to high-resolution structural techniques. Here, we describe the application of single-molecule Förster resonance energy transfer to study the dynamics of integral membrane protein complexes on timescales spanning sub-milliseconds to minutes (10−9–102 s).
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Copyright, Publisher and Additional Information: | © Springer Science+Business Media, LLC, part of Springer Nature 2020. This is an author produced version of a book chapter published in Biophysics of Membrane Proteins: Methods and Protocols. Uploaded in accordance with the publisher's self-archiving policy. | ||||
Keywords: | FRET; SecY; SecA; Protein export; Translocation; Lipid; Liposome | ||||
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Institution: | The University of Leeds | ||||
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Structural Molecular Biology (Leeds) | ||||
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Depositing User: | Symplectic Publications | ||||
Date Deposited: | 12 Apr 2021 11:31 | ||||
Last Modified: | 14 Feb 2023 01:13 | ||||
Status: | Published | ||||
Publisher: | Humana | ||||
Series Name: | Methods in Molecular Biology | ||||
Identification Number: | https://doi.org/10.1007/978-1-0716-0724-4_13 |