Churchill-Angus, A.M., Sedelnikova, S.E. orcid.org/0000-0001-5174-6302, Schofield, T.H.B. et al. (1 more author) (2020) The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and crystallographic analysis. Acta Crystallographica Section F Structural Biology Communications, 76 (12). pp. 577-582. ISSN 2053-230X
Abstract
Tripartite α-pore-forming toxins are constructed of three proteins (A, B and C) and are found in many bacterial pathogens. While structures of the B and C components from Gram-negative bacteria have been described, the structure of the A component of a Gram-negative α-pore-forming toxin has so far proved elusive. SmhA, the A component from the opportunistic human pathogen Serratia marcescens, has been cloned, overexpressed and purified. Crystals were grown of selenomethionine-derivatized protein and anomalous data were collected. Phases were calculated and an initial electron-density map was produced.
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Copyright, Publisher and Additional Information: | © 2020 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | ||||
Keywords: | pore-forming toxin; crystallization; ClyA family; Serratia | ||||
Dates: |
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Institution: | The University of Sheffield | ||||
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) | ||||
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Depositing User: | Symplectic Sheffield | ||||
Date Deposited: | 26 Jan 2021 14:51 | ||||
Last Modified: | 26 Jan 2021 14:51 | ||||
Status: | Published | ||||
Publisher: | International Union of Crystallography (IUCr) | ||||
Refereed: | Yes | ||||
Identification Number: | https://doi.org/10.1107/s2053230x20013862 | ||||
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