Hull, MA orcid.org/0000-0001-7414-1576, Booth, JK, Tisbury, A et al. (4 more authors) (1999) Cyclooxigenase 2 is up-regulated and localized to macrophages in the intestine of Min mice. British Journal of Cancer, 79 (9-10). pp. 1399-1405. ISSN 0007-0920
Abstract
Expression of cyclooxygenase 2 (COX-2) is believed to play an important role in adenoma formation in murine polyposis models, and inhibition of COX-2 activity may, at least, partly explain the chemopreventative activity of non-steroidal anti-inflammatory drugs against colorectal cancer in humans. However, the mechanism by which COX-2 acts in intestinal tumorigenesis remains unresolved because of conflicting data on the cellular localization of COX-2 in intestinal mucosa. Using immunohistochemistry with specific COX-2 antiserum, we have shown that COX-2 protein is localized to interstitial cells at the base of and within adenomas of the small and large intestine of multiple intestinal neoplasia (Min) mice. No COX-2 staining was observed in dysplastic epithelial cells within adenomas or in histologically normal epithelium. Moreover, COX-2 staining was observed in lamina propria cells of histologically normal intestine of Min mice. No staining was demonstrated in wild-type littermates. The rat monoclonal antibody F4/80 was used to show that COX-2-positive cells represented a subset of the macrophage population present in the intestine of Min mice. Localization of COX-2 to macrophages implies a paracrine effect of COX2 function on epithelial cells in adenomas and also on histologically normal epithelium. Up-regulation of COX-2 expression in lamina propria macrophages may precede loss of the second functional Apc allele in epithelial cells before adenoma formation in the Min mouse model of intestinal tumorigenesis.
Metadata
Authors/Creators: |
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Copyright, Publisher and Additional Information: | © Cancer Research Campaign 1999. This work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. |
Keywords: | Adenoma; cyclooxygenase; immunohistochemistry; macrophage; Min mouse; prostaglandin |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds) > Section of Experimental Haematology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 04 Sep 2020 15:04 |
Last Modified: | 04 Sep 2020 15:04 |
Status: | Published |
Publisher: | Churchill Livingstone |
Identification Number: | https://doi.org/10.1038/2Fsj.bjc.6690224 |
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