Liu, X., Sun, B., Kell, R.E.M. et al. (6 more authors) (2018) The Antimicrobial Activity of Mononuclear Ruthenium(II) Complexes Containing the dppz Ligand. ChemPlusChem, 83 (7). pp. 643-650.
Abstract
The cis-α isomer of [Ru(bb7)(dppz)]2+(dppz=dipyrido[3,2-a:2′,3′-c]phenazine; bb7=bis[4(4′-methyl-2,2′-bipyridyl)]-1,7-alkane) has been synthesised. The minimum inhibitory concentrations and the minimum bactericidal concentrations of [Ru(bb7)(dppz)]2+and its parent complex [Ru(phen)2(dppz)]2+(phen=1,10-phenanthroline) were determined against a range of bacteria. The results showed that both ruthenium complexes exhibited good activity against Gram-positive bacteria, but [Ru(bb7)(dppz)]2+showed at least eightfold better activity against the Gram-negative bacteria than [Ru(phen)2(dppz)]2+. Luminescence assays demonstrated that [Ru(bb7)(dppz)]2+accumulated in a Gram-negative bacterium to the same degree as in a Gram-positive species, and assays with liposomes showed that [Ru(bb7)(dppz)]2+interacted more strongly with membranes than the parent [Ru(phen)2(dppz)]2+complex. The DNA binding affinity for [Ru(bb7)(dppz)]2+was determined to be 6.7 × 106m−1. Although more toxic to eukaryotic cells than [Ru(phen)2(dppz)]2+, [Ru(bb7)(dppz)]2+exhibited greater activity against bacteria than eukaryotic cells.
Metadata
Authors/Creators: |
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Copyright, Publisher and Additional Information: | This is the peer reviewed version of the following article: X. Liu, B. Sun, R. E. M. Kell, H. M. Southam, J. A. Butler, X. Li, R. K. Poole, F. R. Keene, J. G. Collins, ChemPlusChem 2018, 83, 643, which has been published in final form at https://doi.org/10.1002/cplu.201800042. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | ||||
Keywords: | antimicrobial agents; bioinorganic chemistry; DNA binding; lipophilicity; ruthenium | ||||
Dates: |
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Institution: | The University of Sheffield | ||||
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) | ||||
Funding Information: |
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Depositing User: | Symplectic Sheffield | ||||
Date Deposited: | 08 Aug 2018 08:19 | ||||
Last Modified: | 30 Mar 2019 01:43 | ||||
Published Version: | https://doi.org/10.1002/cplu.201800042 | ||||
Status: | Published | ||||
Publisher: | Wiley | ||||
Refereed: | Yes | ||||
Identification Number: | https://doi.org/10.1002/cplu.201800042 |