Muthu, V., Eachus, H., Ellis, P. et al. (2 more authors) (2016) Rx3 and Shh direct anisotropic growth and specification in the zebrafish tuberal/anterior hypothalamus. Development, 143 (14). pp. 2651-2663. ISSN 0950-1991
Abstract
In the developing brain, growth and differentiation are intimately linked. Here we show that in the zebrafish embryo, the homeodomain transcription factor rx3 co-ordinates these processes to build the tuberal/anterior hypothalamus. Analysis of rx3 chk mutant/rx3 morphant fish and EdU pulse-chase studies reveal that rx3 is required to select tuberal/anterior hypothalamic progenitors and to orchestrate their anisotropic growth. In the absence of rx3 function, progenitors accumulate in the 3(rd) ventricular wall, die or are inappropriately-specified, the shh+ anterior recess does not form, and its resident pomc+, ff1b+ and otp+ TH+ cells fail to differentiate. Manipulation of Shh signalling shows that shh co-ordinates progenitor cell selection and behaviour by acting as an on-off switch for rx3 Together our studies show that shh and rx3 govern formation of a distinct progenitor domain that elaborates pattern through its anisotropic growth and differentiation.
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Copyright, Publisher and Additional Information: | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. | ||||||
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Institution: | The University of Sheffield | ||||||
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) | ||||||
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Depositing User: | Symplectic Sheffield | ||||||
Date Deposited: | 07 Jul 2016 13:47 | ||||||
Last Modified: | 25 Oct 2018 13:30 | ||||||
Published Version: | http://dx.doi.org/10.1242/dev.138305 | ||||||
Status: | Published | ||||||
Publisher: | Company of Biologists | ||||||
Refereed: | Yes | ||||||
Identification Number: | https://doi.org/10.1242/dev.138305 | ||||||
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