White Rose University Consortium logo
University of Leeds logo University of Sheffield logo York University logo

Electrochemical detection of K+-evoked quantal secretory events from isolated rat type I carotid body cells

Hatton, C.J. and Peers, C. (1997) Electrochemical detection of K+-evoked quantal secretory events from isolated rat type I carotid body cells. Experimental Physiology, 82 (2). pp. 415-418. ISSN 0958-0670

Full text not available from this repository.

Abstract

Using amperometric techniques, electrochemical events associated with vesicular transmitter release were recorded from isolated rat type I carotid body cells when exposed to a solution containing 50mm K+. Events were enhanced in amplitude by preloading cells with the catecholamine precursor, L-b-3,4-dihydroxyphenylalanine (L-DOPA). K+-evoked secretion was abolished by the non-selective Ca2, channel blocker Cd2+ (100 ,/M) and markedly reduced by the L-type Ca2+ channel blocker nifedipine (5 µM). Our results indicate that secretion from isolated rat type I cells can be monitored electrochemically and we demonstrate a major role for L-type Ca2+ channels in mediating K+-evoked secretion.

Item Type: Article
Copyright, Publisher and Additional Information: © 1997 The Physiological Society. Paper freely available from the Experimental Physiology website.
Academic Units: The University of Leeds > Faculty of Medicine and Health (Leeds) > Leeds Institute of Genetics, Health and Therapeutics (LIGHT) > Academic Unit of Cardiovascular Medicine (Leeds)
The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Genetics, Health and Therapeutics (LIGHT) > Academic Unit of Cardiovascular Medicine (Leeds)
Depositing User: Repository Officer
Date Deposited: 28 Sep 2007 09:16
Last Modified: 29 Sep 2010 14:20
Published Version: http://ep.physoc.org/cgi/reprint/82/2/415.pdf
Status: Published
Publisher: Blackwell Publishing
Refereed: Yes
URI: http://eprints.whiterose.ac.uk/id/eprint/3381

Actions (login required)

View Item View Item