Hatton, C.J. and Peers, C. (1997) Electrochemical detection of K+-evoked quantal secretory events from isolated rat type I carotid body cells. Experimental Physiology, 82 (2). pp. 415-418. ISSN 0958-0670
Using amperometric techniques, electrochemical events associated with vesicular transmitter release were recorded from isolated rat type I carotid body cells when exposed to a solution containing 50mm K+. Events were enhanced in amplitude by preloading cells with the catecholamine precursor, L-b-3,4-dihydroxyphenylalanine (L-DOPA). K+-evoked secretion was abolished by the non-selective Ca2, channel blocker Cd2+ (100 ,/M) and markedly reduced by the L-type Ca2+ channel blocker nifedipine (5 µM). Our results indicate that secretion from isolated rat type I cells can be monitored electrochemically and we demonstrate a major role for L-type Ca2+ channels in mediating K+-evoked secretion.
|Copyright, Publisher and Additional Information:||© 1997 The Physiological Society. Paper freely available from the Experimental Physiology website.|
|Institution:||The University of Leeds|
|Academic Units:||The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Genetics, Health and Therapeutics (LIGHT) > Academic Unit of Cardiovascular Medicine (Leeds)|
|Depositing User:||Repository Officer|
|Date Deposited:||28 Sep 2007 09:16|
|Last Modified:||18 Jun 2015 17:25|