Extraction of extracellular polymeric substances (EPS) from red soils (Ultisols)

Abstract

Extracellular polymeric substances (EPS) have many beneficial functions in soils. Accurate quantification of EPS in soils is crucial. Here, five methods were compared for their suitability for extraction of EPS from Ultisols: hot water extractable polysaccharide (HWEP), hot dilute acid extractable polysaccharide (HDAEP), easily extractable glomalin (EEG), sodium sulfide (SS) and cation exchange resin (CER) method. Humic-acid equivalent (HAE) was used as an indicator for extracellular contamination and ATP for quantifying intracellular contamination from cell lysis. Among the tested methods, CER resulted in EPS extraction with minimal contamination. Therefore, we propose that CER is currently the most appropriate method for extraction of EPS from Ultisols.

Metadata

Authors/Creators:	Wang, S Redmile-Gordon, M Mortimer, M Cai, P Wu, Y Peacock, CL https://orcid.org/0000-0003-3754-9294 Gao, C Huang, Q
Copyright, Publisher and Additional Information:	© 2019 Elsevier Ltd. This is an author produced version of a paper published in Soil Biology and Biochemistry. Uploaded in accordance with the publisher's self-archiving policy.
Keywords:	Soil biofilms; Extracellular polymeric substances; Polysaccharide; Cation exchange resin; Easily extractable glomalin
Dates:	Accepted: 16 May 2019 Published (online): 20 May 2019 Published: August 2019
Institution:	The University of Leeds
Academic Units:	The University of Leeds > Faculty of Environment (Leeds) > School of Earth and Environment (Leeds) > Earth Surface Science Institute (ESSI) (Leeds)
Depositing User:	Symplectic Publications
Date Deposited:	06 Jun 2019 10:52
Last Modified:	20 May 2020 00:38
Status:	Published
Publisher:	Elsevier Ltd.
Identification Number:	https://doi.org/10.1016/j.soilbio.2019.05.014