Ali, F., Aute, S., Sreedharan, S. et al. (5 more authors) (2018) Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe. Chemical Communications, 54 (15). pp. 1849-1852. ISSN 1359-7345
Abstract
BODIPY derivative, SF-1, exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically detects HOCl generated in these organelles. SF-1 is compatible with 3D-SIM imaging of individual cells.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 Royal Society of Chemistry. This is an author-produced version of a paper accepted for publication in Chemical Communications. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 07 Feb 2018 12:37 |
Last Modified: | 17 Dec 2020 17:45 |
Status: | Published |
Publisher: | Royal Society of Chemistry |
Refereed: | Yes |
Identification Number: | 10.1039/c7cc09433g |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:127139 |