Costa, CL, López-Ureña, D, de Oliveira Assis, T et al. (12 more authors) (2016) A MLST Clade 2 Clostridium difficile strain with a variant TcdB induces severe inflammatory and oxidative response associated with mucosal disruption. Anaerobe, 40. pp. 76-84. ISSN 1075-9964
Abstract
The epidemiology of Clostridium difficile infections is highly dynamic as new strains continue to emerge worldwide. Here we present a detailed analysis of a new C. difficile strain (ICC-45) recovered from a cancer patient in Brazil that died from severe diarrhea. A polyphasic approach assigned a new PCR-ribotype and PFGE macrorestriction pattern to strain ICC-45, which is toxigenic (tcdA+, tcdB+ and ctdB+) and classified as ST41 from MLST Clade 2 and toxinotype IXb. Strain ICC-45 encodes for a variant TcdB that induces a distinct CPE in agreement with its toxinotype. Unlike epidemic NAP1/027 strains, which are also classified to MLST Clade 2, strain ICC-45 is susceptible to fluoroquinolones and does not overproduce toxins TcdA and TcdB. However, supernatants from strain ICC-45 and a NAP1/027 strain produced similar expression of pro-inflammatory cytokines, epithelial damage, and oxidative stress response in the mouse ileal loop model. These results highlight inflammation and oxidative stress as common features in the pathogenesis of C. difficile Clade 2 strains. Finally, this work contributes to the description of differences in virulence among various C. difficile strains.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016, Elsevier Ltd. All rights reserved. This is an author produced version of a paper accepted for publication in Anaerobe. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Clostridium difficile; MLST Clade 2; Variant toxin B; Inflammation; Oxidative stress response |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds) > Section of Molecular Gastroenterology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 05 Aug 2016 11:41 |
Last Modified: | 22 Jul 2017 04:06 |
Published Version: | http://10.0.3.248/j.anaerobe.2016.06.005 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.anaerobe.2016.06.005 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:102940 |