Ingram, R., Tagoh, H., Riggs, A.D. and Bonifer, C. (2005) Rapid, solid-phase based automated analysis of chromatin structure and transcription factor occupancy in living eukaryotic cells. Nucleic Acids Research, 33 (1). e1-e1. ISSN 1362-4962
Transcription factors, chromatin components and chromatin modification activities are involved in many diseases including cancer. However, the means by which alterations in these factors influence the epigenotype of specific cell types is poorly understood. One problem that limits progress is that regulatory regions of eukaryotic genes sometimes extend over large regions of DNA. To improve chromatin structure–function analysis over such large regions, we have developed an automated, relatively simple procedure that uses magnetic beads and a capillary sequencer for ligation-mediated-PCR (LM-PCR). We show that the procedure can be used for the rapid examination of chromatin fine-structure, nucleosome positioning as well as changes in transcription factor binding-site occupancy during cellular differentiation.
|Copyright, Publisher and Additional Information:||© 2005, the authors. Published in Nucleic Acids Research, Oxford University Press. Supplementary material available at http://nar.oxfordjournals.org/cgi/reprint/33/1/e1|
|Institution:||The University of Leeds|
|Academic Units:||The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds) > Section of Genetics (Leeds)|
|Depositing User:||Repository Officer|
|Date Deposited:||13 Mar 2006|
|Last Modified:||16 Sep 2016 18:59|