Rapid, solid-phase based automated analysis of chromatin structure and transcription factor occupancy in living eukaryotic cells

Ingram, R., Tagoh, H., Riggs, A.D. and Bonifer, C. (2005) Rapid, solid-phase based automated analysis of chromatin structure and transcription factor occupancy in living eukaryotic cells. Nucleic Acids Research, 33 (1). e1-e1. ISSN 1362-4962

Full text available as:

Preview

Text
boniferc2.pdf
Available under License : See the attached licence file.
Download (802Kb)

Published Version: http://nar.oxfordjournals.org/cgi/reprint/33/1/e1

Abstract

Transcription factors, chromatin components and chromatin modification activities are involved in many diseases including cancer. However, the means by which alterations in these factors influence the epigenotype of specific cell types is poorly understood. One problem that limits progress is that regulatory regions of eukaryotic genes sometimes extend over large regions of DNA. To improve chromatin structure–function analysis over such large regions, we have developed an automated, relatively simple procedure that uses magnetic beads and a capillary sequencer for ligation-mediated-PCR (LM-PCR). We show that the procedure can be used for the rapid examination of chromatin fine-structure, nucleosome positioning as well as changes in transcription factor binding-site occupancy during cellular differentiation.

Item Type:	Article
Copyright, Publisher and Additional Information:	© 2005, the authors. Published in Nucleic Acids Research, Oxford University Press. Supplementary material available at http://nar.oxfordjournals.org/cgi/reprint/33/1/e1
Academic Units:	The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds) > Section of Genetics (Leeds)
Depositing User:	Repository Officer
Date Deposited:	13 Mar 2006
Last Modified:	08 Feb 2013 17:02
Published Version:	http://nar.oxfordjournals.org/cgi/reprint/33/1/e1
Status:	Published
Refereed:	Yes
Identification Number:	10.1093/nar/gni001
URI:	http://eprints.whiterose.ac.uk/id/eprint/967

Actions (login required)

View Item