Gigliobianco, G., Chong, C.K. and MacNeil, S. (2015) Simple surface coating of electrospun poly-L-lactic acid scaffolds to induce angiogenesis. Journal of Biomaterials Applications.
Abstract
Tissue-engineered constructs often fail due to poor integration with the patient's tissues. Specifically, they fail to be neovascularised, leading to the death and loss of the implanted tissues. Thus, there is a need to produce angiogenic materials to improve tissue integration. We describe the development of a layer-by-layer approach to coat electrospun scaffolds to help promote angiogenesis into these biomaterials once implanted. Electrospun poly-L-lactic acid was coated comparing two different techniques - one using alternative layers of polyethyleneImine (PEI) and polyacrylic Acid (PAC) and one with alternative layers of PEI and heparin for a total of seven layers in both cases. Both scaffolds were then coated with heparin as the final layer. The scaffold coated with alternate PEI and PAC showed a clear ability to bind the most heparin. This scaffold was then studied further for its ability to bind vascular endothelial growth factor, which was confirmed using an ELISA. The scaffold coated with seven alternate layers of PEI and PAC and heparin was then implanted in a chick chorionic allantoic membrane (CAM) assay. After a period of 7 days in the CAM, the coated scaffold showed strong angiogenic activity. In contrast, the uncoated scaffolds did not promote angiogenesis. We conclude that this approach to functionalising scaffolds is effective within a clinically relevant time period (7 days in an in-vivo angiogenic model) and suggest this will be useful for improving integration of scaffolds once implanted.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015 Sage. This is an author produced version of a paper subsequently published in Journal of Biomaterials Applications . Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | ELISA; Poly-L-lactic acid; chick chorionic allantoic membrane; electrospinning; heparin; layer-by-layer; plasma polymerisation; vascular endothelial growth factor |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 02 Jun 2015 14:47 |
Last Modified: | 08 Nov 2016 19:25 |
Published Version: | http://dx.doi.org/10.1177/0885328215569891 |
Status: | Published |
Publisher: | SAGE Publications |
Refereed: | Yes |
Identification Number: | 10.1177/0885328215569891 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:86406 |