Bailey, R.G., Turner, R.D., Mullin, N. et al. (3 more authors) (2014) The Interplay between Cell Wall Mechanical Properties and the Cell Cycle in Staphylococcus aureus. Biophysical Journal, 107 (11). 2538 - 2545. ISSN 0006-3495
Abstract
The nanoscale mechanical properties of live Staphylococcus aureus cells during different phases of growth were studied by atomic force microscopy. Indentation to different depths provided access to both local cell wall mechanical properties and whole-cell properties, including a component related to cell turgor pressure. Local cell wall properties were found to change in a characteristic manner throughout the division cycle. Splitting of the cell into two daughter cells followed a local softening of the cell wall along the division circumference, with the cell wall on either side of the division circumference becoming stiffer. Once exposed, the newly formed septum was found to be stiffer than the surrounding, older cell wall. Deeper indentations, which were affected by cell turgor pressure, did not show a change in stiffness throughout the division cycle, implying that enzymatic cell wall remodeling and local variations in wall properties are responsible for the evolution of cell shape through division.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2014 Biophysical Society. Reproduced in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Physics and Astronomy (Sheffield) The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Molecular Biology and Biotechnology (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 09 Feb 2015 09:42 |
Last Modified: | 23 Jun 2023 21:44 |
Published Version: | http://dx.doi.org/10.1016/j.bpj.2014.10.036 |
Status: | Published |
Publisher: | Biophysical Society |
Refereed: | Yes |
Identification Number: | 10.1016/j.bpj.2014.10.036 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:83310 |