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Intraspecfic variation in cold-temperature metabolic phenotypes of Arabidopsis lyrata ssp petraea

Davey, M.P., Woodward, F.I. and Quick, W.P. (2009) Intraspecfic variation in cold-temperature metabolic phenotypes of Arabidopsis lyrata ssp petraea. Metabolomics, 5 (1). pp. 138-149. ISSN 1573-3882

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Abstract

Atmospheric temperature is a key factor in determining the distribution of a plant species. Alongside this, plant populations growing at the margin of their range may exhibit traits that indicate genetic differentiation and adaptation to their local abiotic environment. We investigated whether geographically separated marginal populations of Arabidopsis lyrata ssp. petraea have distinct metabolic phenotypes associated with exposure to cold temperatures. Seeds of A. petraea were obtained from populations along a latitudinal gradient, namely Wales, Sweden and Iceland and grown in a controlled cabinet environment. Mannose, glucose, fructose, sucrose and raffinose concentrations were different between cold treatments and populations, especially in the Welsh population, but polyhydric alcohol concentrations were not. The free amino acid compositions were population specific, with fold differences in most amino acids, especially in the Icelandic populations, with gross changes in amino acids, particularly those associated with glutamine metabolism. Metabolic fingerprints and profiles were obtained. Principal component analysis (PCA) of metabolite fingerprints revealed metabolic characteristic phenotypes for each population and temperature. It is suggested that amino acids and carbohydrates were responsible for discriminating populations within the PCA. Metabolite fingerprinting and profiling has proved to be sufficiently sensitive to identify metabolic differences between plant populations at different atmospheric temperatures. These findings show that there is significant natural variation in cold metabolism among populations of A. l. petraea which may signify plant adaptation to local climates.

Item Type: Article
Copyright, Publisher and Additional Information: © 2009 Springer. This is an author produced version of a paper subsequently published in Metabolomics. Uploaded in accordance with the publisher's self-archiving policy.
Keywords: Arabidopsis lyrata spp. petraea; Cold; Direct injection mass spectrometry; Environmental metabolomics; Metabolic phenotypes; Metabolite fingerprinting
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Science (Sheffield) > School of Biological Sciences (Sheffield) > Department of Animal and Plant Sciences (Sheffield)
Depositing User: Miss Anthea Tucker
Date Deposited: 23 Mar 2009 17:53
Last Modified: 08 Feb 2013 16:58
Published Version: http://dx.doi.org/10.1007/s11306-008-0127-1
Status: Published
Publisher: Springer Verlag
Refereed: Yes
Identification Number: 10.1007/s11306-008-0127-1
URI: http://eprints.whiterose.ac.uk/id/eprint/7979

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