Cohen, N., Soen, Y. and Braun, E. (1998) Spatio-temporal dynamics of networks of heart cells in culture. Physica A, 249 (1). pp. 600-604. ISSN 0378-4371
Spontaneous activity of cultured rat ventricular heart cells is studied as a function of cell density and ambient temperature. Three complementary methods are used: calcium fluorescence microscopy (monitoring intracellular free Ca2+ concentrations), real-time motion detection and electrophysiology. Statistical features of beating activity in single cells, small groups of cells and large monolayered networks are characterized and compared. At relatively high temperatures, a gradual transition from stochastic activity at the level of a single cell to regular synchronized beating in large, dense networks is found. Lowering the temperature results in a gradual loss of synchronization and the emergence of spatio-temporal dynamics, expressed by bursts of activity and contractions on many length and time scales.
|Institution:||The University of Leeds|
|Academic Units:||The University of Leeds > Faculty of Engineering (Leeds) > School of Computing (Leeds)|
|Depositing User:||Miss Jamie Grant|
|Date Deposited:||12 Mar 2009 18:55|
|Last Modified:||04 Nov 2016 04:09|