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Spatio-temporal dynamics of networks of heart cells in culture

Cohen, N., Soen, Y. and Braun, E. (1998) Spatio-temporal dynamics of networks of heart cells in culture. Physica A, 249 (1). pp. 600-604. ISSN 0378-4371


Spontaneous activity of cultured rat ventricular heart cells is studied as a function of cell density and ambient temperature. Three complementary methods are used: calcium fluorescence microscopy (monitoring intracellular free Ca2+ concentrations), real-time motion detection and electrophysiology. Statistical features of beating activity in single cells, small groups of cells and large monolayered networks are characterized and compared. At relatively high temperatures, a gradual transition from stochastic activity at the level of a single cell to regular synchronized beating in large, dense networks is found. Lowering the temperature results in a gradual loss of synchronization and the emergence of spatio-temporal dynamics, expressed by bursts of activity and contractions on many length and time scales.

Item Type: Article
Institution: The University of Leeds
Academic Units: The University of Leeds > Faculty of Engineering (Leeds) > School of Computing (Leeds)
Depositing User: Miss Jamie Grant
Date Deposited: 12 Mar 2009 18:55
Last Modified: 15 Sep 2014 01:38
Published Version: http://dx.doi.org/10.1016/S0378-4371(97)00521-9
Status: Published
Publisher: Elsevier Science
Identification Number: 10.1016/S0378-4371(97)00521-9
URI: http://eprints.whiterose.ac.uk/id/eprint/7966

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