Ding, Yanning, Brackenbury, William J. orcid.org/0000-0001-6882-3351, Onganer, Pinar U. et al. (4 more authors) (2008) Epidermal growth factor upregulates motility of Mat-LyLu rat prostate cancer cells partially via voltage-gated Na+ channel activity. Journal of cellular physiology. pp. 77-81. ISSN 0021-9541
Abstract
The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage-gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. Similar treatment with EGF significantly and dose-dependently enhanced the cells' migration through Transwell filters. Both the patch clamp recordings and the migration assay suggested that endogenous EGF played a similar role. Importantly, co-application of EGF and tetrodotoxin, a highly selective VGSC blocker, abolished 65% of the potentiating effect of EGF. It is suggested that a significant portion of the EGF-induced enhancement of migration occurred via VGSC activity.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Copyright © 2007 Wiley-Liss, Inc. This is an author produced version of a paper published in Journal of Cellular Physiology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | FACTOR RECEPTOR, BREAST-CANCER, IN-VITRO, METASTATIC CASCADE, SODIUM-CHANNELS, LUNG-CANCER, FUNCTIONAL EXPRESSION, MEMBRANE-ACTIVITY, ELECTRIC-FIELD, LINE |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 29 Apr 2014 15:00 |
Last Modified: | 21 Apr 2024 23:05 |
Published Version: | https://doi.org/10.1002/jcp.21289 |
Status: | Published |
Refereed: | Yes |
Identification Number: | https://doi.org/10.1002/jcp.21289 |
Related URLs: |