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A secretory pathway-localised cation diffusion facilitator confers plant manganese tolerance

Peiter, E., Montanini, B., Gobert, A., Pedas, P., Husted, S., Maathuis, F.J.M., Blaudez, D., Chalot, M. and Sanders, D. (2006) A secretory pathway-localised cation diffusion facilitator confers plant manganese tolerance. Proceedings of the National Academy of Sciences of the United States of America, 104 (20). pp. 8532-8537. ISSN 0027-8424

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Abstract

Manganese toxicity is a major problem for plant growth in acidic soils, but cellular mechanisms that facilitate growth in such conditions have not been clearly delineated. Established mechanisms that counter metal toxicity in plants involve chelation and cytoplasmic export of the metal across the plasma or vacuolar membranes out of the cell or sequestered into a large organelle, respectively. We report here that expression of the Arabidopsis and poplar MTP11 cation diffusion facilitators in a manganese-hypersensitive yeast mutant restores manganese tolerance to wild-type levels. Microsomes from yeast expressing AtMTP11 exhibit enhanced manganese uptake. In accord with a presumed function of MTP11 in manganese tolerance, Arabidopsis mtp11 mutants are hypersensitive to elevated levels of manganese, whereas plants overexpressing MTP11 are hypertolerant. In contrast, sensitivity to manganese deficiency is slightly decreased in mutants and increased in overexpressing lines. Promoter-GUS studies showed that AtMTP11 is most highly expressed in root tips, shoot margins, and hydathodes, but not in epidermal cells and trichomes, which are generally associated with manganese accumulation. Surprisingly, imaging of MTP11–EYFP fusions demonstrated that MTP11 localizes neither to the plasma membrane nor to the vacuole, but to a punctate endomembrane compartment that largely coincides with the distribution of the trans-Golgi marker sialyl transferase. Golgi-based manganese accumulation might therefore result in manganese tolerance through vesicular trafficking and exocytosis. In accord with this proposal, Arabidopsis mtp11 mutants exhibit enhanced manganese concentrations in shoots and roots. We propose that Golgi-mediated exocytosis comprises a conserved mechanism for heavy metal tolerance in plants.

Item Type: Article
Institution: The University of York
Academic Units: The University of York > Biology (York)
Depositing User: York RAE Import
Date Deposited: 17 Feb 2009 10:40
Last Modified: 17 Feb 2009 10:40
Published Version: http://dx.doi.org/10.1073/pnas.0609507104
Status: Published
Publisher: National Academy of Sciences; 1999
Identification Number: 10.1073/pnas.0609507104
URI: http://eprints.whiterose.ac.uk/id/eprint/7402

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