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Active root-inhabiting microbes identified by rapid incorporation of plant-derived carbon into RNA

Vandenkoornhuyse, P., Mahé, S., Ineson, P., Staddon, P., Ostle, N., Cliquet, J., Francez, A., Fitter, A.H. and Young, J.P.Y. (2007) Active root-inhabiting microbes identified by rapid incorporation of plant-derived carbon into RNA. Proceedings of the National Academy of Sciences USA, 104 (43). 16970-16975 16970. ISSN 0027-8424

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Plant roots harbor a large diversity of microorganisms that have an essential role in ecosystem functioning. To better understand the level of intimacy of root-inhabiting microbes such as arbuscular mycorrhizal fungi and bacteria, we provided 13CO2 to plants at atmospheric concentration during a 5-h pulse. We expected microbes dependent on a carbon flux from their host plant to become rapidly labeled. We showed that a wide variety of microbes occurred in roots, mostly previously unknown. Strikingly, the greatest part of this unsuspected diversity corresponded to active primary consumers. We found 17 bacterial phylotypes co-occurring within roots of a single plant, including five potentially new phylotypes. Fourteen phylotypes were heavily labeled with the 13C. Eight were phylogenetically close to Burkholderiales, which encompass known symbionts; the others were potentially new bacterial root symbionts. By analyzing unlabeled and 13C-enriched RNAs, we demonstrated differential activity in C consumption among these root-inhabiting microbes. Arbuscular mycorrhizal fungal RNAs were heavily labeled, confirming the high carbon flux from the plant to the fungal compartment, but some of the fungi present appeared to be much more active than others. The results presented here reveal the possibility of uncharacterized root symbioses.

Item Type: Article
Institution: The University of York
Academic Units: The University of York > Biology (York)
Depositing User: York RAE Import
Date Deposited: 18 Feb 2009 09:22
Last Modified: 18 Feb 2009 09:22
Published Version: http://dx.doi.org/10.1073/pnas.0705902104
Status: Published
Publisher: National Academy of Sciences; 1999
Identification Number: 10.1073/pnas.0705902104
URI: http://eprints.whiterose.ac.uk/id/eprint/7389

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