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Win1, a transcriptional activator of epidermal wax accumulation in Arabidopsis

Broun, P., Poindexter, P., Osborne, E., Jiang, Cai-Zhong and Riechmann, J. L. (2004) Win1, a transcriptional activator of epidermal wax accumulation in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 101 (13). pp. 4706-4711. ISSN 0027-8424

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Abstract

Epicuticular wax forms a layer of hydrophobic material on plant aerial organs, which constitutes a protective barrier between the plant and its environment. We report here the identification of WIN1, an Arabidopsis thaliana ethylene response factor-type transcription factor, which can activate wax deposition in overexpressing plants. We constitutively expressed WIN1 in transgenic Arabidopsis plants, and found that leaf epidermal wax accumulation was up to 4.5-fold higher in these plants than in control plants. A significant increase was also found in stems. Interestingly, ≈50% of the additional wax could only be released by complete lipid extractions, suggesting that not all of the wax is superficial. Gene expression analysis indicated that a number of genes, such as CER1, KCS1, and CER2, which are known to be involved in wax biosynthesis, were induced in WIN1 overexpressors. This observation indicates that induction of wax accumulation in transgenic plants is probably mediated through an increase in the expression of genes encoding enzymes of the wax biosynthesis pathway.

Item Type: Article
Institution: The University of York
Academic Units: The University of York > Biology (York)
Depositing User: York RAE Import
Date Deposited: 16 Mar 2009 15:07
Last Modified: 16 Mar 2009 15:07
Published Version: http://dx.doi.org/10.1073/pnas.0305574101
Status: Published
Publisher: National Academy of Sciences; 1999
Refereed: Yes
Identification Number: 10.1073/pnas.0305574101
URI: http://eprints.whiterose.ac.uk/id/eprint/7308

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