Baumann, C.G., Morris, D.G., Sreenan, J.M. and Leese, H.J. (2007) The quiet embryo hypothesis: molecular characteristics favouring viability. Molecular Reproduction and Development, 74 (10). pp. 1345-1353. ISSN 1040-452X
It has been proposed that the viability of early mammalian embryos is associated with a metabolism that is quiet rather than active (Leese HJ, 2002:BioEssays 24:845-849). The data on which this hypothesis was based were largely drawn from measurements on the depletion and appearance of amino acids from the culture medium. Data on the de novo synthesis of protein in in vivo- and in vitro-derived bovine embryos, as determined from the flux of radiolabeled methionine, have provided further support of the hypothesis and are interpreted to provide a new set of testable propositions that could illuminate the molecular basis of the quiet metabolism phenotype. The propositions are based on the premise that the extent of DNA damage, and the RNA and protein content of the immature oocyte, are key factors in determining whether the zygote progresses to the blastocyst stage. We propose that stochastic events and environmental stresses determine whether the condition of the genome, transcriptome, and proteome of the zygote will support development. Several molecular components are identified that may determine the viability of a zygote, and we speculate that the cellular response to unfavorable events or excessive DNA damage may be the premature activation of the embryonic genome and of apoptosis.
|Institution:||The University of York|
|Academic Units:||The University of York > Biology (York)|
|Depositing User:||York RAE Import|
|Date Deposited:||20 Mar 2009 14:41|
|Last Modified:||20 Mar 2009 14:41|
|Publisher:||John Wiley & Sons|